Maintain breeding records of the animal properly like when the animal came to heat, when it was inseminated, designation of the person who inseminated the animals etc.

Provide balanced ration and feed to the animals.

Mineral mixture should be included in the ration @2 % of the ration.

Provide clean water to the animals.

Keep animals free from the environmental stressors like heat stress by providing shade and cool environment to the animal during summer by planting, painting water bathing and wallowing areas etc.

Overfeeding of animals should be avoided.

Maintain hygienic conditions around animals.

Do not feed infested grains and green fodder and soiled wheat straw to animals.

Signs of estrus should be observed regularly and the animals showing signs of estrus should be taken to trained personnel for the insemination within 12 hours.

Underweight animals should not be inseminated.

Avoid overcrowding, especially at the time of AI.

Inseminate the animals showing signs of estrus.

Animals with congenital defects of ovary, fallopian tubes or uterus should not be inseminated. Such animals should be culled immediately as they will transmit the diseases to the next generation.

Cows showing abnormal cervical mucus discharge should not be inseminated and should be checked for any uterine infections.

Do not put the semen straws back into the liquid nitrogen cylinder once it has been taken out. It should be either used or discarded.

AI guns should be used aseptically to avoid infection and contamination.

Maintain the breeding record properly to check the inbreeding.

Do not use the same bull for AI with his own daughter to prevent inbreeding.

Rotate the breeding bull among the dairy farmers and check the record after every 3rd year to check inbreeding.

Rule out the anatomical causes by per-rectal examination.

Blood test to know the hormonal status of the animal.

Rule out the infection by per-rectal examination, blood test and history of the animals.

Genetic tests or pedigree records of the animals may be recommended in some cases to rule out the genetic causes.

Hormonal therapy may be used if required.